Vijay Setaluri, Ph. D

Cell Culture Core

Skin diseases constitute a major public health burden in the US and worldwide. Understanding the cellular and molecular basis of these disease is facilitated by the availability of quality controlled pure populations of skin cells derived from both healthy and affected individuals. The Cell Culture Core of the UW SDRC will serve a large interdisciplinary group of scientists in their studies of human skin biology in health and disease. The Core Leader for the Cell Culture Core is Vijay Setaluri, PhD. Dr. Setaluri seeks to understand molecular mechanisms of skin cancer melanoma tumorigenesis and progression, and basic cell and molecular biology of epidermal melanocytes.

The specific aims of the Cell Culture Core are:

  1. Establish and maintain a cost-effective one-stop facility as a reliable source of quality-assured cells and cell cultures from normal and pathological human skin specimens and mouse models of skin diseases.
  2. Generate and maintain cryo-preserved stocks of induced pluripotent stem cells (iPSCs) from skin fibroblasts for differentiation into epidermal and other cell types.
  3. Produce quality controlled high-titer lentiviruses for transduction of primary cell cultures.
  4. Provide hands-on training for manipulation of primary skin cells in vitro. This includes training in liposome-mediated transfection, electroporation, viral transduction, differentiation of iPSCs, generation of three-dimensional skin constructs.
  5. Provide freshly grown primary cultures and frozen cell pellets for isolation, purification, and analysis of macromolecules for biochemical and molecular biological studies.

Request Services

Item
Unit
SDRC Member Price*
 Primary Cells (keratinocyte, melanocyte, fibroblast): Live
 (T-25)
 $75.00
 Primary Cells (keratinocyte, melanocyte, fibroblast): Frozen Vial
 (1mL vial)
 $100.00
 Primary Cells (keratinocyte, melanocyte, fibroblast): Frozen Pellet
 (1x106cells)  $50.00
 Established Cell Lines (except iPSC): Live
 (T-25)
 $25.00
 Established Cell Lines (except iPSC): Frozen Vial
 (1mL vial)
 $50.00
 Established Cell Lines (except iPSC): Frozen Pellet
 (1x106cells)  $25.00
 Lentivirus production  (1x109 CFU/ml)  $300.00
 Mycoplasma testing (24 hour turnaround)
 (per sample)
 $25.00
 Specialty cell culture medium preparation
 (500ml)
 $150.00
Training
 Lentivirus

 (Fees vary based on the need. Please contact Hannah Strelchenko to discuss your project.)

 Electroporation

 *For non-member prices, and for any services or products not listed in the schedule, please contact Program Manager Hannah Strelchenko.

Published work that includes data obtained through Cell Culture Core services should cite the following:

"Research reported in this publication was supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health (NIH) under Award Number P30 AR066524, Skin Diseases Research Center at the University of Wisconsin. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH."

Please contact Hannah Strelchenko with any questions: sdrc@dermatology.wisc.edu or (608) 263-9366.