Jack Longley, MD

Experimental Cutaneous Pathology Core

Skin disease causes significant morbidity, economic loss, and mortality to the US population, regardless of age, gender, ethnicity or socio-economic group. A lack of appropriately prepared skin samples and expertise in processing and interpreting both human and animal skin are barriers to skin disease research. The Experimental Cutaneous Pathology Core will increase access to all types of skin samples and skin-specific pathology expertise, thereby benefitting the population of the US.

The Core Leader for the Experimental Cutaneous Pathology Core is B. Jack Longley, MD. Dr. Longley has over 30 years of experience in diagnostic and experimental pathology involving both human and animal skin. He is the director of the UW Dermatopathology Laboratory, which functions with the Mohs Surgical Laboratory.

The specific aims of the Experimental Cutaneous Pathology Core are:

  1. Provide samples of normal human skin and a wide variety of diseased skin, including de-identified specimens that can be obtained for feasibility studies exempt from IRB review.
  2. Provide subsidized pathology services including histologic, histochemical, immuno-histochemical, and immuno-fluorescence techniques optimized for studying human and animal skin.
  3. Provide hands on training and help with access to and application of state of the art, molecular and microscopic techniques to morphologic and quantitative studies of skin specimens.

Current Services:

The Experimental Cutaneous Pathology Core (ECPC) offers a variety of services available to researchers interested in skin biology. We have samples of normal human skin, as well as samples of various human skin diseases, including de-identified specimens. In addition to formalin-fixed paraffin-embedded tissue samples, the ECPC also offers fresh tissue samples. Please see the list of services below for more information or see our fee schedule.

Histology, Immunohistochemistry/In situ Hybridization

  • Routine histology services ranging from specimen processing & embedding to special stains
  • Immunohistochemistry and in situ hybridization for new and established antigens, with standardization of results through automation
  • Normal and benign formalin-fixed paraffin-embedded control tissues
  • To order tissue slides and samples, fill out the Tissue Request Form and send to sdrc@dermatology.wisc.edu
  • To order IHC services, fill out the IHC Request Form and send to sdrc@dermatology.wisc.edu

Tissue Microarray (TMA) Construction

  • TMAs from formalin-fixed, paraffin-embedded tissue blocks constructed to research needs. **Requests for TMA construction require consultation, please submit a scientific basis for your project, a brief description of what your project needs are and your source of funding. Send this information to Hannah Strelchenko at sdrc@dermatology.wisc.edu

COMING SOON: Laser Capture Microscopy (LCM)

  • Laser microdissection of tissue specimens to isolate cells/DNA/RNA of interest

Pathologist Services:

The ECPC offers access to expert pathologists: Core Director B. Jack Longley, MD and Core Co-Director, Ruth Sullivan, VMD, PhD.

Dr. Sullivan offers technical and professional support for histologic analysis of animal models of human disease, especially rodent models. She will provide animal pathology consulting services, including diagnostic evaluation, in concert with Dr. Longley. Together they hold their slide review sessions for UW SDRC members on the 2nd and 4th Wednesdays of the month at 9:00 a.m. at WIMR 2, Room 6525.

Dr. Sullivan also holds monthly open slide reviews for the UW Carbone Cancer Center on the last Monday of the month at 1pm at WIMR 2, Room 6525. All are welcome to attend those sessions.

inForm® Tissue Finder™ Software Reservations:

The Experimental Cutaneous Pathology Core offers access on a reservation basis to the latest version of inForm® Tissue Finder™, Advanced Image Analysis Software from PerkinElmer for research pathology.

  • Our software station is located in the ECPC laboratory, Room 402 SMI. This software is used to analyze images obtained from any Nuance™ or Vectra™ multispectral imaging system.
  • To use this software, you must be trained and designated by your PI as an authorized user and complete the access request form
  • Once authorization has been granted, you may reserve time for self-service use of the software. Authorized users can access the software reservation system by clicking this link (you will be required to use your UW NetID). The scheduler sends notification of your reserved time to the email address linked to your NetID. You will be billed for the time you reserve, rounded to the nearest quarter hour based on your login times. Hourly rates are listed in the table below.

We offer training sessions and hands-on technical assistance to researchers interested in using the software. Please send any requests for training and assistance to Hannah Strelchenko at sdrc@dermatology.wisc.edu.

 Hourly rates:

inForm® Tissue Finder™ software Non UW SDRC Member   UW SDRC Member
Self-Service $26/hour $18.20/Hour
Assistance provided by ECPC personnel $35/hour $35/hour
Assistance provided by pathologist $100/hour $100/hour

Published work that includes data obtained through Experimental Cutaneous Pathology Core services should cite the following:

"Research reported in this publication was supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health (NIH) under Award Number P30 AR066524, Skin Diseases Research Center at the University of Wisconsin. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH."